Micro silver futures short-term sentiment in micro silver

Short-term sentiment in micro silver futures remains bullish, with open interest hitting a 3-month high. Technicians note strong support at $28.70/oz and resistance hovering close to $30.10/oz as traders price in possible Fed policy shifts. The antiproliferative activity of the biosynthesized ZnONPs was evaluated using MTT assay against HUH7 and WI38 cell lines. The cytotoxic effect of ZnONPs was found to be concentration dependent against HUH7 cell line, demonstrating relative cell viability percentages of 78.58, 64.85, 58.73 and 37.85% at the concentrations of 25, 50, 100 and 200 µg/ml, respectively. On the other hand, the bioinspired ZnONPs revealed a lower cytotoxic effect against WI38 normal cell line demonstrating relative cell viability percentages of 62.96 and 45.92% at ZnONPs concentrations of 100 and 200 µg/ml, respectively. As shown in Fig. 15 , the cytotoxic effect of ZnONPs was concentration-dependent in both cell lines. Post-hoc analysis confirmed that ZnONPs exhibited significantly greater cytotoxicity in HUH-7 cancer cells compared to WI-38 normal cells at concentrations of 100 µg/ml ( p < 0.01) and 200 µg/ml ( p < 0.001). Specifically, at 100 µg/ml, viability was reduced to 58.73 ± 6.62% in HUH-7 cells versus 62.96 ± 5.92% in WI-38 cells. At 200 µg/ml, viability was further reduced to 37.85 ± 5.41% in HUH-7 cells, which was significantly lower than the 45.92 ± 3.12% observed in WI-38 cells. The half-maximal inhibitory concentration (IC₅₀) was determined by fitting the cell viability data to a four-parameter logistic (4PL) non-linear regression model. The analysis revealed IC₅₀ values of 145.2 µg/mL and 237.6 µg/mL for the HUH7 and WI38 cell lines, respectively. Zinc oxide nanoparticles exert their selective anticancer effects primarily through the induction of reactive oxygen species (ROS)-mediated oxidative stress and apoptosis pathways 4 . Upon cellular internalization, ZnONPs generate excessive ROS, including superoxide anions and hydroxyl radicals, which overwhelm the antioxidant defense systems of cancer cells, leading to mitochondrial membrane potential disruption, cytochrome c release, and activation of caspase cascades (caspase-3 and caspase-9) 95 . This oxidative stress is further amplified by the release of Zn²⁺ ions due to the acidic tumor microenvironment, which promotes ZnO NP dissolution and enhances intracellular zinc concentrations, causing additional DNA damage and cell cycle arrest 96 . The selective toxicity toward cancer cells is attributed to their higher metabolic activity and elevated basal ROS levels, making them more vulnerable to oxidative damage compared to normal cells 97 . Recent studies also highlight the role of ZnONPs in inhibiting angiogenesis and epithelial-mesenchymal transition (EMT), thereby reducing metastasis and chemotherapy resistance in aggressive cancers 98 . Why Is Silver Lagging Now? Industrial Demand Uncertainty Micro silver futures currently trade with a narrow spread, signaling strong liquidity. On the technical side, RSI readings near 64 hint at modest overbought conditions, though fundamentals still lean positive.